| 產品編號 | bs-1063R |
| 英文名稱 | ADRB3 Rabbit pAb |
| 中文名稱 | β3腎上腺素能受體抗體 |
| 別 名 | beta 3 Adrenergic Receptor; beta3-adrenergic receptor; beta3-AR; adrenergic; beta-3-receptor; ADR B3; ADRB 3; ADRB3R; Adrenergic beta 3 receptor; B3AR; beta 3 adrenoceptor; beta 3 adrenoreceptor; beta 3AR; B3AR; BETA3AR; ADRB3_HUMAN. |
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Specific References (7) | bs-1063R has been referenced in 7 publications.
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| 研究領域 | 心血管 細胞生物 神經生物學 生長因子和激素 細胞膜受體 內分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Sheep,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADRB3: 310-408/408 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein. Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed mainly in adipose tissues. Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily. SWISS: P13945 Gene ID: 155 Database links: Entrez Gene: 155 Human Entrez Gene: 11556 Mouse SwissProt: P13945 Human SwissProt: P25962 Mouse Unigene: 2549 Human Unigene: 278475 Mouse Unigene: 10100 Rat Omim: 109691 HumanARβ3是新近發現的,繼β1和β2之后的第3個β型腎上腺素受體亞型.分子量：44KDa(404AA) 近年來研究表明:β3-腎上腺素受體該受體基因突變或基因多態性與機體肥胖及心血管疾病相關;心力衰竭時該受體的含量明顯增加,β3腎上腺素能受體與心血管疾病的相關研究可能為心血管疾病的預測、防治的新方法. |
| 產品圖片 |
Sample:
K562(Human) Cell Lysate at 40 ug
Primary: Anti-beta 3 Adrenergic Receptor (bs-1063R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated(bs-1063R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
