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Dnmt3a Rabbit pAb (bs-0497R)  
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產品編號 bs-0497R
英文名稱 Dnmt3a Rabbit pAb
中文名稱 DNA甲基轉移酶-3α抗體
別    名 DNA(cytosine 5 ) methyltransferase 3 alpha; DNA(cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a.  
Specific References  (7)     |     bs-0497R has been referenced in 7 publications.
[IF=8.739] Ai Wei. et al. Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis. 2021 Aug 11  WB,IHC ;  Mouse.  
[IF=8.401] Dr. xingren Chen. et al. Reversal of epigenetic PPARγ suppression by diacerein alleviates oxidative stress and osteoarthritis in mice. 2022 Feb 24  WB ;  Mouse.  
[IF=5.714] Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261.  WB ;  Human.  
[IF=5.2] Zhao, Qian, et al. "Prenatal Cocaine Exposure Impairs Cognitive Function of Progeny Via Insulin Growth Factor II Epigenetic Regulation." Neurobiology of Disease (2015).  WB ;  Mouse.  
[IF=3.85] Wu, Yuting, et al. "Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis." Toxicology and Applied Pharmacology 315 (2017): 35-49.  WB ;  Mouse.  
[IF=3.337] Jiachen Wang. et al. Upregulation of miR-137 Expression Suppresses Tumor Growth and Progression via Interacting with DNMT3a Through Inhibiting the PTEN/Akt Signaling in HCC. Oncotargets Ther. 2021; 14: 165–176  WB ;  Human.  
[IF=0] G?kmen, Esra, and Kemal Ergin. "DNA Methyltransferase Expression and Proliferation Status of Metastatic Breast Cancer Cell Line After Prolonged and Repeated Rapamycin and Melatonin Application." Meandros Medical Journal.  Human.  
研究領域 腫瘤  細胞生物  信號轉導  細胞凋亡  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Cow)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 100 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Dnmt3a: 26-100/912 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]

Function:
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Subunit:
Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4).

Subcellular Location:
Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.

Tissue Specificity:
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Post-translational modifications:
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.

Similarity:
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.

SWISS:
Q9Y6K1

Gene ID:
1788

Database links:

Entrez Gene: 1788 Human

Entrez Gene: 13435 Mouse

Omim: 602769 Human

SwissProt: Q9Y6K1 Human

SwissProt: O88508 Mouse

Unigene: 515840 Human

Unigene: 5001 Mouse



腫瘤組織存在DNA甲基化紊亂,包括與細胞增殖周期密切相關的癌基因低甲基化和抑癌基因高甲基化DNA甲基轉移酶(Dnmt)參與甲基化的形成(主要是Dnmt3a和Dnmt3b)和維持(主要是Dnmt1)
產品圖片
Sample: A549(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Lovo(Human) Cell Lysate at 30 ug Primary: Anti-Dnmt3a (bs-0497R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 120 kD
Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: Testis (Rat) Lysate at 40 ug Lane 4: 293T (Human) Cell Lysate at 30 ug Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 6: Molt-4 (Human) Cell Lysate at 30 ug Lane 7: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Dnmt3a (bs-0497R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD
Sample: Embryo(Mouse) Lysate at 40 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-Dnmt3a (bs-0497R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 120 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dnmt3a) Polyclonal Antibody, Unconjugated (bs-0497R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated(bs-0497R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated(bs-0497R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-Dnmt3a antibody (bs-0497R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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