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IASPP Rabbit pAb (bs-0284R)  
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產(chǎn)品編號 bs-0284R
英文名稱 IASPP Rabbit pAb
中文名稱 凋亡抑制因子IASPP抗體
別    名 IASPP; inhibitor of apoptosis stimulating protein of p53; Inhibitor of ASPP protein; NFkB interacting protein 1; NKIP1; PPP1R13B-like protein; PPP1R13L; Protein iASPP; protein phosphatase 1 regulatory(inhibitor) subunit 13 like; RAI; RelA-associated inhibitor; IASPP_HUMAN; RelA-associated inhibitor; Inhibitor of ASPP protein; NFkB-interacting protein 1.  
Specific References  (1)     |     bs-0284R has been referenced in 1 publications.
[IF=3.182] Wu F et al. Elevated Expression of Inhibitor of Apoptosis-stimulating Protein of p53 (iASPP) and Methyltransferase-like 3 (METTL3) Correlate with Poor Prognosis in FIGO Ib1-IIa Squamous Cell Cervical Cancer. J Cancer. 2020 Feb 10;11(9):2382-2389.  IHC,WB ;  human.  
研究領(lǐng)域 腫瘤  免疫學(xué)  染色質(zhì)和核信號  信號轉(zhuǎn)導(dǎo)  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Rat (predicted: Mouse,Sheep,Cow,Dog,GuineaPig)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 89 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 細(xì)胞外基質(zhì) 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IASPP: 15-100/828 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 iASPP(inhibitor of apoptosis stimulating protein of p53)regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. Belongs to the ASPP family.

Function:
Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.

Subunit:
Interacts with RELA NF-kappa-B subunit and with SP1 via its C-terminus part. Interacts with p53/TP53, TP63 and TP73.

Subcellular Location:
Cytoplasm. Nucleus. Note=Predominantly cytoplasmic but also nuclear.

Tissue Specificity:
Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte.

Similarity:
Belongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain.

SWISS:
Q8WUF5

Gene ID:
10848

Database links:

Entrez Gene: 10848 Human

Entrez Gene: 333654 Mouse

Omim: 607463 Human

SwissProt: Q8WUF5 Human

SwissProt: Q3TCU2 Mouse

SwissProt: Q5I1X5 Mouse

Unigene: 466937 Human



IASPP(inhibitory member of the ASPP family) 是新近發(fā)現(xiàn)的高度保守的p53相關(guān)基因, 是細(xì)胞凋亡ASPP家族的另一個成員。
iASPP能夠抑制ASPP1,2的促凋亡作用,其蛋白產(chǎn)物定位于細(xì)胞核內(nèi),具有結(jié)合NF-κB p65亞基和p53功能,進(jìn)而抑制NF-κB的轉(zhuǎn)錄調(diào)節(jié)和p53對凋亡的調(diào)節(jié)功能。
研究者發(fā)現(xiàn)iASPP蛋白,能夠干擾p53基因發(fā)揮正常的促使細(xì)胞死亡的作用。這就意味著iASPP在正常細(xì)胞轉(zhuǎn)化為癌癥細(xì)胞方面起著重要的促進(jìn)作用,即iASPP具有促進(jìn)癌變的功能。表達(dá)位置:細(xì)胞漿,部分細(xì)胞核。
inhibitor of ASPP

產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IASPP) Polyclonal Antibody, Unconjugated (bs-0284R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IASPP) polyclonal Antibody, Unconjugated (bs-0284R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): HL60 (blue). Primary Antibody (green line): Rabbit Anti-IASPP antibody (bs-0284R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-IASPP antibody (bs-0284R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:A431. Primary Antibody (green line): Rabbit Anti-IASPP antibody (bs-0284R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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