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CNTF Rabbit pAb (bs-1272R)  
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產品編號 bs-1272R
英文名稱 CNTF Rabbit pAb
中文名稱 睫狀神經營養因子抗體
別    名 Ciliary Neurotrophic Factor; CNTF; CNTF_HUMAN; HCNTF.  
Specific References  (4)     |     bs-1272R has been referenced in 4 publications.
[IF=5.32] Yang, Xu, et al. "Reversal of Bone Cancer Pain by HSV-1-Mediated Silencing of CNTF in an Afferent Area of the Spinal Cord Associated with AKT-ERK Signal Inhibition." Current Gene Therapy 14.5 (2014): 377-388.  
[IF=3.244] Fu XM et al. The?Combination?of?Adipose-derived?Schwann-like?Cells?and?Acellular?Nerve?Allografts?PromotesSciatic?Nerve?Regeneration?and?Repair?through the?JAK2/STAT3?Signaling Pathway in Rats. Neuroscience. 2019 Nov 1;422:134-145.  ICF&WB ;  Rat.  
[IF=2.784] Xiong?LL et al. Tree shrew neural stem cell transplantation promotes functional recovery of tree shrews with a hemi?sectioned spinal cord injury by upregulating nerve growth factor expression. Int J Mol Med. 2018 Jun;41(6):3267-3277.  IF ;  tree shrews.  
[IF=1.94] De Nan. et al. In Vitro Study of Adipose-Derived Mesenchymal Stem Cells Transduced with Lentiviral Vector Carrying the Brain-Derived Neurotrophic Factor Gene. Int J Stem Cells. 2020; 13(3): 386–393  WB ;  Mouse.  
研究領域 發育生物學  神經生物學  干細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat,Rabbit (predicted: Mouse,Pig)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CNTF: 21-150/200 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition to the predominant monocistronic transcript originating from this locus, the gene is also transcribed as a readthrough transcript with the upstream Zfp91 gene. The resulting protein is an isoform of Zfp91 and does not include Cntf sequence. Readthrough transcription of Zfp91 and Cntf has been observed in human and mouse.

Function:
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Subunit:
Belongs to the CNTF family.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Nervous system.

Similarity:
Belongs to the CNTF family.

SWISS:
Q6NTB0

Gene ID:
1270

Database links:

Entrez Gene: 1270 Human

Entrez Gene: 12803 Mouse

Entrez Gene: 25707 Rat

Omim: 118945 Human

SwissProt: P26441 Human

SwissProt: Q6NTB0 Human

SwissProt: P51642 Mouse

SwissProt: P20294 Rat

Unigene: 524920 Human

Unigene: 715806 Human

Unigene: 290924 Mouse

Unigene: 6067 Rat



神經干細胞標志物
有學者認為:睫狀神經營養因子能促進運動神經軸突再生,CNTF可以阻止損傷后的運動神經元的退形性變性,目前CNTF對神經營養作用還在深入的研究中。
產品圖片
Tissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated(bs-1272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated(bs-1272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat spinal cord tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated(bs-1272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CNTF Polyclonal Antibody, Unconjugated(bs-1272R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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