| 產品編號 | bs-1436R |
| 英文名稱 | PBP Rabbit pAb |
| 中文名稱 | 磷脂酰乙醇胺結合蛋白1抗體 |
| 別 名 | phosphatidylethanolamine-binding protein; PEBP; PEBP 1; PBP; RKIP; Phosphatidylethanolamine-binding protein 1; PEBP-1; PEBP1; Prostatic-binding protein; Prostatic binding protein; Neuropolypeptide h3; HCNPpp; Raf kinase inhibitor protein; Hippocampal cholinergic neurostimulating peptide; HCNP. |
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Specific References (2) | bs-1436R has been referenced in 2 publications.
[IF=1.581] Ping et al. Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations. (2015) Int.J.Clin.Exp.Pathol. 8:10164-70 IHC ; Human.
[IF=1.39] Wei et al. Promoter methylation and expression of Raf kinase inhibitory protein in esophageal squamous cell carcinoma. (2017) Oncol.Lett. 13:1866-1872 IHC-P ; Human.
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| 研究領域 | 腫瘤 細胞生物 神經生物學 信號轉導 細胞周期蛋白 轉錄調節因子 激酶和磷酸酶 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RKIP: 51-187/187 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. [SUBCELLULAR LOCATION] Cytoplasm. Belongs to the phosphatidylethanolamine-binding protein family. Function: Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. Subcellular Location: Cytoplasm. Similarity: Belongs to the phosphatidylethanolamine-binding protein family. SWISS: P30086 Gene ID: 5037 Database links: Entrez Gene: 5037 Human Entrez Gene: 23980 Mouse Omim: 604591 Human SwissProt: P30086 Human SwissProt: P70296 Mouse Unigene: 433863 Human Unigene: 195898 Mouse Unigene: 338476 Mouse Unigene: 473809 Mouse Unigene: 29745 Rat 信號傳導（Signaling Intermediates） Raf激酶抑制蛋白(PKIP)屬于磷脂酰乙醇胺結合蛋白 （phosphatidylethanolamine-binding protein, PEBP）家族,廣泛存在于各種不同的生物中,PKIP還參與了對NF-kB信號通路和G蛋白偶聯受體信號通路的調控,參與NF-κB信號通路的調控,參與了對細胞內多種信號轉導通路的調節作用。RKIP在膜的生物合成、精子發生、神經發育和細胞凋亡等生理過程中發揮重要作用，并參與了老年癡呆癥及糖尿病等的病理過程。有學者認為：RKIP有抑制腫瘤細胞轉移的作用。 |
| 產品圖片 |
Sample: Brain(mouse) at lysates;
Primary: Anti-PBP (bs-1436R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:21 kD
Observed band size:21 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RKIP/PBP Polyclonal Antibody, Unconjugated(bs-1436R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
