| 產(chǎn)品編號 | bs-1444R |
| 英文名稱 | TLR3 Rabbit pAb |
| 中文名稱 | Toll樣受體3抗體 |
| 別 名 | Toll-like receptor 3; TLR3; CD283; CD283 antigen; TLR 3; Toll Like Receptor 3; TLR3_HUMAN. |
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Specific References (6) | bs-1444R has been referenced in 6 publications.
[IF=5.246] Zou Xiong. et al. Toll-Like Receptors Serve as Biomarkers for Early Diagnosis and Prognosis Assessment of Kidney Renal Clear Cell Carcinoma by Influencing the Immune Microenvironment: Comprehensive Bioinformatics Analysis Combined With Experimental Validation. Front Mol Biosci. 2022 Jan;0:24 FC ; Human.
[IF=4.6] Wang, Shaolan, et al. "Xenobiotic receptor PXR regulates innate immunity via activation of NLRP3 inflammasome in vascular endothelial cells." Journal of Biological Chemistry (2014): jbc-M114. WB ; Human.
[IF=4.03] Zhu, Wenhua, et al. "Pristane induces autophagy in macrophages, promoting a STAT1-IRF1-TLR3 pathway and arthritis." Clinical Immunology (2016). WB ; Rat.
[IF=3.751] Milara et al. Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist. (2015) Respir.Res. 16:12 IHC,WB ; Human.
[IF=3.152] Zhao R et al. Activation of Toll-like receptor 3 promotes pathological corneal neovascularization by enhancement of SDF-1-mediated endothelial progenitor cell recruitment. (2018) Exp Eye Res. 178:177-185. IF ; Mouse.
[IF=2.2] Zhu, W., et al. "Overexpression of Toll?\Like Receptor 3 in Spleen is Associated with Experimental Arthritis in Rats."Scandinavian Journal of Immunology 76.3 (2012): 263-270.. WB ; Rat.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號 微生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 100 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TLR3: 801-905/905 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. Function: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Subunit: Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with TICAM1. Homodimer formation is triggered by ligand binding and is required for TLR3 signaling. Binding of double-stranded RNA is required for the interaction with SRC. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane. Tissue Specificity: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. Post-translational modifications: Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. DISEASE: Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. Similarity: Belongs to the Toll-like receptor family. Contains 22 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. Contains 1 TIR domain. SWISS: O15455 Gene ID: 7098 Database links: Entrez Gene: 7098 Human Entrez Gene: 142980 Mouse Omim: 603029 Human SwissProt: O15455 Human SwissProt: Q99MB1 Mouse Unigene: 657724 Human Unigene: 33874 Mouse Toll樣受體3(TLR3)作為一種重要跨膜信號轉(zhuǎn)導(dǎo)受體參 與了內(nèi)毒素誘發(fā)炎癥反應(yīng)的病理過程. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Jurkat (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: Molt-4 (Human) Cell Lysate at 30 ug
Primary: Anti-TLR3 (bs-1444R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 120 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TLR3) Polyclonal Antibody, Unconjugated (bs-1444R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TLR3 Polyclonal Antibody, Unconjugated(bs-1444R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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