| 產品編號 | bs-1524R |
| 英文名稱 | GlyR alpha 1 + 2 + 3 Rabbit pAb |
| 中文名稱 | 甘氨酸受體alpha 1/2/3型抗體 |
| 別 名 | glycine receptor alpha 1; glycine receptor alpha 2; glycine receptor alpha 3; GLRA1; GLRA2; Glycine receptor 48 kDa subunit; Glycine receptor alpha 2; Glycine receptor subunit alpha 3; Glycine receptor subunit alpha 2; Glycine receptor, alpha 1 subunit; Glycine receptor, alpha 2 subunit; MGC138878; MGC138879; STHE; alpha 1 Glycine Receptor; GLRA1; GLRA1_HUMAN; Glycine receptor 48 kDa subunit; Glycine receptor alpha 1; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1; Glycine receptor, alpha 1 subunit; HKPX1; STHE; GLR; Glra2; GLRA2_HUMAN; Glycine receptor alpha 2 polypeptide; Glycine receptor alpha 2 subunit; Glycine receptor subunit alpha 2; Glycine receptor subunit alpha-2; Gycine receptor alpha 2. |
| 研究領域 | 免疫學 神經生物學 通道蛋白 細胞膜受體 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GlyR alpha 1: 101-200/449 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain. Subcellular Location: Cell Membrane SWISS: Q6DJV9 Gene ID: 2742 Database links: Entrez Gene: 2741 Human Entrez Gene: 14654 Mouse Omim: 138491 Human SwissProt: P23415 Human SwissProt: Q64018 Mouse Unigene: 121490 Human Unigene: 89320 Mouse Unigene: 10109 Rat 甘氨酸受體alpha 1是中樞神經系統中的重要抑制性離子通道受體，是哺乳類中樞神經系統中神經遞質受體蛋白，屬于配體門控離子通道超家族成員. |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Lung tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Cerebellum tissue lysates
Lane 7: Rat Testis tissue lysates
Lane 8: Human HeLa cell lysates
Lane 9: Human A431 cell lysates
Lane 10: Human SH-SY5Y cell lysates
Primary: Anti-GlyR alpha 1 + 2 + 3 (bs-1524R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 53 kDa
Sample:
Spinal cord (Mouse) Lysate at 40 ug
Spinal cord (Rat) Lysate at 40 ug
Primary: Anti-GlyR alpha 1 + 2 + 3 (bs-1524R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 54 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GlyR alpha 1/2/3 Polyclonal Antibody, Unconjugated(bs-1524R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
