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ABCG4 Rabbit pAb (bs-1727R)  
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產品編號 bs-1727R
英文名稱 ABCG4 Rabbit pAb
中文名稱 ABC膜轉運蛋白抗體
別    名 ATP binding cassette, subfamily G member 4; ABCG 4; ATP binding cassette subfamily G member 4; WHITE 2; WHITE2; ABCG4_HUMAN.  
Specific References  (2)     |     bs-1727R has been referenced in 2 publications.
[IF=3.05] Matsumoto, Koichi, et al. "Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood–cerebrospinal fluid barrier in human brain." Histochemistry and Cell Biology (2015): 1-15.  IHC-P ;  Human.  
[IF=1.22] Ueno, Masaki, et al. "Blood–brain barrier and blood–cerebrospinal fluid barrier in normal and pathological conditions." Brain Tumor Pathology (2016): 1-8.  IHC-P ;  Human.  
研究領域 免疫學  神經生物學  轉運蛋白  細胞表面分子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Rabbit,Pig,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 71 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ABCG4: 551-646/646 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 he ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes. ABCG4 protein is highly expressed in both human and mouse brain, it is a 646aa molecule in human (chr 11q23) and mouse. It is an integral membrane protein may be involved in macrophage lipid homeostasis. The ABCG4 protein’s abundant expression in brain and close evolutionary relationship to the other members of the subfamily suggests a potential role in cholesterol transport.

Function:
May be involved in macrophage lipid homeostasis.

Subcellular Location:
Membrane; Multi-pass membrane protein (Probable).

Similarity:
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.

SWISS:
Q9H172

Gene ID:
64137

Database links:

Entrez Gene: 64137 Human

Entrez Gene: 192663 Mouse

Entrez Gene: 300664 Rat

Omim: 607784 Human

SwissProt: Q9H172 Human

Unigene: 126378 Human

Unigene: 101876 Mouse

Unigene: 29008 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ABCG4) Polyclonal Antibody, Unconjugated (bs-1727R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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