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Neurokinin A Rabbit pAb (bs-0069R)  
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產品編號 bs-0069R
英文名稱 Neurokinin A Rabbit pAb
中文名稱 神經激肽A抗體
別    名 Neurokinin alpha; Neurokinin K; NeurokininA; NeurokininK; Neuromedin L; NeuromedinL; Neuropeptide gamma; Neuropeptide K; NeuropeptideK; NK A; NKA; NKN A; NKNA; NPK; PPT; Preprotachykinin 1; Preprotachykinin1; Protachykinin 1 precursor; Protachykinin1 precursor; Substance K; SubstanceK; TAC 1; TAC I; TAC1; TACI; TKN 1; TKN1; neurokinin A/neuropeptide K.  
研究領域 神經生物學  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Chicken,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 11/13 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human neurokinin A/neuropeptide K: 98-107/129 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] This anitbody is crose activity with Neurokinin B.

Function:
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Subcellular Location:
Secreted.

Similarity:
Belongs to the tachykinin family.

SWISS:
P20366

Gene ID:
6863

Database links:

Entrez Gene: 6863 Human

Entrez Gene: 21333 Mouse

Entrez Gene: 24806 Rat

Omim: 162320 Human

SwissProt: P20366 Human

SwissProt: P41539 Mouse

SwissProt: P06767 Rat

Unigene: 2563 Human

Unigene: 1920 Rat



產品圖片
Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Stomach tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human U-2 OS cell lysates Lane 5: Human SH-SY5Y cell lysates Lane 6: Human u251 cell lysates Primary: Anti-Neurokinin A (bs-0069R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 11/13 kDa Observed band size: 11 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated(bs-0069R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated(bs-0069R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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