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HES5 Rabbit pAb (bs-3767R)  
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產品編號 bs-3767R
英文名稱 HES5 Rabbit pAb
中文名稱 發狀分裂相關增強子-5抗體
別    名 BHLHB38; Transcription factor HES-5; Class B basic helix-loop-helix protein 38(bHLHb38); Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876; HES5_HUMAN.  
Specific References  (2)     |     bs-3767R has been referenced in 2 publications.
[IF=6.119] Liu D et al. PNO1, which is negatively regulated by miR-340-5p, promotes lung adenocarcinoma progression through Notch signaling pathway. Oncogenesis. 2020 Jun 1;9(5):58.  WB ;  Human.  
[IF=2.96] Zhang, Haihua, et al. "Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway." Biochimie (2016).  WB ;  Other Species.  
研究領域 信號轉導  干細胞  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 18 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Hes5: 21-120/166 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008]

Function:
Transcriptional repressor of genes that require a bHLH protein for their transcription

Subunit:
Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in fetal heart and brain tumors.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.

SWISS:
Q5TA89

Gene ID:
388585

Database links:

Entrez Gene: 388585 Human

Entrez Gene: 15208 Mouse

Entrez Gene: 79225 Rat

SwissProt: Q5TA89 Human

SwissProt: P70120 Mouse

SwissProt: Q03062 Rat



產品圖片
Sample: Kidney (Rat) Lysate at 40 ug Primary: Anti-alpha smooth muscle Actin (bs-3767R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HES5) Polyclonal Antibody, Unconjugated (bs-3767R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HES5) Polyclonal Antibody, Unconjugated (bs-3767R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Hes5 Polyclonal Antibody, Unconjugated(bs-3767R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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