| 產品編號 | bs-3311R |
| 英文名稱 | Phospho-Numb (Ser276) Rabbit pAb |
| 中文名稱 | 磷酸化膜相關蛋白Numb抗體 |
| 別 名 | Numb(phospho S276); Numb(phospho Ser276); p-Numb(Ser276); c14_5527; C14orf41; FLJ31314; h Numb; h-Numb; Numb; Numb homolog(Drosophila); Numb homolog; Numb protein homolog; NUMB_HUMAN; Protein numb homolog; Protein S171; S171. |
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Specific References (1) | bs-3311R has been referenced in 1 publications.
[IF=5.37] Mah, In Kyoung, et al. "Atypical PKC-iota Controls Stem Cell Expansion via Regulation of the Notch Pathway." Stem Cell Reports (2015). WB ; Mouse.
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| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 免疫學 神經生物學 細胞膜受體 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Numb around the phosphorylation site of Ser276: QG(p-S)FR |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Function: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Subunit: Interacts with CDH1 and TFAP2B (By similarity). Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. Subcellular Location: Membrane; Peripheral membrane protein. Post-translational modifications: Phosphorylated on Ser-276 and Ser-295 by CaMK1. Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation. Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. Similarity: Contains 1 PID domain. SWISS: P49757 Gene ID: 8650 Database links: Entrez Gene: 8650 Human Entrez Gene: 18222 Mouse Omim: 603728 Human SwissProt: P49757 Human SwissProt: Q9QZS3 Mouse Unigene: 654609 Human Unigene: 714879 Human Unigene: 4390 Mouse Unigene: 102078 Rat 膜相關蛋白Numb是細胞命運決定因子：在細胞有絲分裂期，Numb選擇性地分布于細胞的一側,在胞質分裂后則被分配于一個子細胞，Numb通過抑制跨膜受體Notch而發揮作用, Numb信號途徑在前體細胞的神經元調控方面起著重要作用. |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-Phospho-Numb (Ser276) (bs-3311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 75 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
