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phospho-Cdc6 (Ser54) Rabbit pAb (bs-5246R)  
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產品編號 bs-5246R
英文名稱 phospho-Cdc6 (Ser54) Rabbit pAb
中文名稱 磷酸化細胞分裂周期蛋白6抗體
別    名 Cell Division Cycle protein 6; Cdc 18L; Cdc 6; CDC18(cell division cycle 18, S.pombe, homolog) like; CDC18(S.pombe); CDC18 like; CDC18(S.pombe); Cdc18L; CDC6 related protein; Cdc6p; Cell cycle controller; Cell division control protein 6; Cell division control protein 6 homolog; Cell division cycle 18; Cell division cycle 18 homolog; Cell division cycle 6 homolog; Cell division cycle 6 protein; HsCDC 18; HsCDC 6; HsCDC18; HsCDC6; p62; p62(cdc 6); p62(cdc6); CDC6_HUMAN.  
產品類型 磷酸化抗體 
研究領域 細胞生物  染色質和核信號  細胞周期蛋白  轉錄調節因子  細胞分化  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Dog,Horse)
產品應用 ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 63 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Cdc6 around the phosphorylation site of Ser54: PL(p-S)PR 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Function:
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

Subunit:
Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1.

Subcellular Location:
Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells.

DISEASE:
Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5) [MIM:613805]. MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal.

Similarity:
Belongs to the CDC6/cdc18 family.

SWISS:
Q99741

Gene ID:
990

Database links:

Entrez Gene: 990 Human

Omim: 602627 Human

SwissProt: Q99741 Human

Unigene: 405958 Human



細胞分裂周期蛋白6也是細胞周期調節蛋白,是真核生物DNA復制的主要調控因子,參與構成DNA前復制復合物。
產品圖片
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-Cdc6 (Ser54)) polyclonal Antibody, Unconjugated (bs-5246R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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