| 產品編號 | bs-4226R |
| 英文名稱 | VAPA Rabbit pAb |
| 中文名稱 | 囊泡相關膜蛋白相關蛋白A抗體 |
| 別 名 | VAMP A; VAMP associated protein A; VAP 33; VAP A; VAP33; Vesicle associated membrane protein associated protein A; VAPA_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VAPA: 101-200/249 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
VAPA is a type IV membrane protein, present in the plasma membrane, intracellular vesicles and may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Two transcript variants encoding distinct isoforms have been identified. Function: May play a role in vesicle trafficking. Subunit: Homodimer, and heterodimer with VAPB. Interacts with VAMP1, VAMP2, STX1A, BET1, SEC22C and with the C-terminal domain of OCLN. Interacts with OSBPL1A. Interacts (via MSP domain) with ZFYVE27; may retain ZFYVE27 in the endoplasmic reticulum and regulate its function in cell projections formation. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Tissue Specificity: Ubiquitous. Similarity: Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. Contains 1 MSP domain. SWISS: Q9P0L0 Gene ID: 9218 Database links: Entrez Gene: 9218 Human Entrez Gene: 30960 Mouse Omim: 605703 Human SwissProt: Q9P0L0 Human SwissProt: Q9WV55 Mouse Unigene: 165195 Human Unigene: 266767 Mouse Unigene: 162275 Rat |
| 產品圖片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-VAPA (bs-4226R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
