| 產(chǎn)品編號 | bs-4277R |
| 英文名稱 | ENPP7 Rabbit pAb |
| 中文名稱 | 堿性鞘磷脂酶7抗體 |
| 別 名 | ALK SMase; Alk-SMase; Alkaline sphingomyelin phosphodiesterase; E-NPP 7; Ectonucleotide pyrophosphatase/phosphodiesterase 7; Ectonucleotide pyrophosphatase/phosphodiesterase family member 7; ENPP7; ENPP7_HUMAN; Intestinal alkaline sphingomyelinase; NPP-7. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 染色質(zhì)和核信號 細胞周期蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ENPP7: 351-458/458 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Function: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Note=Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex. Tissue Specificity: Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. Post-translational modifications: N-glycosylated; required for activity and transport to the plasma membrane. Similarity: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. SWISS: Q6UWV6 Gene ID: 339221 Database links: Entrez Gene: 339221 Human Entrez Gene: 238011 Mouse SwissProt: Q6UWV6 Human Unigene: 114084 Human Unigene: 244114 Mouse Unigene: 211790 Rat |
| 產(chǎn)品圖片 |
Sample: HT29 (human)Cell Lysate at 40 ug
Primary: Anti- ENPP7 (bs-4277R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample: FHC (human)Cell Lysate at 40 ug
Primary: Anti- ENPP7 (bs-4277R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 48 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
