| 產(chǎn)品編號 | bs-6064R |
| 英文名稱 | PRSS8 Rabbit pAb |
| 中文名稱 | 絲氨酸蛋白酶8抗體 |
| 別 名 | CAP1; Channel Activating Protease 1; mCAP1; Prostasin; Prostasin heavy chain; protease, serine, 8; Prss8; PRSS8_HUMAN; Serine protease 8. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 激酶和磷酸酶 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30/33 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRSS8: 65-165/343 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PRSS8 is a member of the trypsin family of serine proteases. It can be found in seminal fluid and is highly expressed in prostate epithelia. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. The carboxyterminal region contains a GPI anchor sequence, which anchors prostasin to the cell surface. Phospholipase activity is thought to liberate prostasin from the cell, and cleavage of the linker region also releases prostasin. PRSS8 has been proposed as a marker for ovarian cancer, where it is up-regulated, a marker for androgen-refractory prostate cancer, where it is down-regulated, and as an inhibitor of invasiveness in breast cancer. Function: Possesses a trypsin-like cleavage specificity. Subunit: Heterodimer of two chains, light and heavy, held by a disulfide bond. Subcellular Location: Prostasin: Cell membrane; Single-pass membrane protein. Prostasin light chain: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus. Prostasin heavy chain: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus. Tissue Specificity: Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: Q16651 Gene ID: 5652 Database links: Entrez Gene: 5652 Human Entrez Gene: 76560 Mouse Omim: 600823 Human SwissProt: Q16651 Human SwissProt: Q9ESD1 Mouse Unigene: 75799 Human Unigene: 5875 Mouse Unigene: 107364 Rat |
| 產(chǎn)品圖片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-PRSS8 (bs-6064R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30/33 kD
Observed band size: 40/43 kD
Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Muscle (Rat) Lysate at 40 ug
Primary:
Anti- PRSS8 (bs-6064R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37-40 kD
Observed band size: 40 kD
Tissue/cell: human prostate carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PRSS8 Polyclonal Antibody, Unconjugated(bs-6064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PRSS8 Polyclonal Antibody, Unconjugated(bs-6064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
