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Acid Phosphatase Rabbit pAb (bs-6291R)  
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產(chǎn)品編號 bs-6291R
英文名稱 Acid Phosphatase Rabbit pAb
中文名稱 酸性磷酸酶抗體
別    名 Acid phosphatase 1 soluble; Acid phosphatase of erythrocyte; Adipocyte acid phosphatase; Cytoplasmic phosphotyrosyl protein phosphatase; HAAP; Low molecular weight phosphotyrosine protein phosphatase; PAP1; PAP2; Protein tyrosine phosphatase; PTPase; Purple acid phosphatase; Red cell acid phosphatase 1; PPAC_HUMAN.  
研究領(lǐng)域 腫瘤  細胞生物  細胞周期蛋白  激酶和磷酸酶  腫瘤細胞生物標志物  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 18 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Acid Phosphatase: 71-158/158 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Phosphatase enzymes catalyse hydrolysis of phosphoric acid esters of various alcohols, e.g. a hexose phosphate, to yield the alcohol and free inorganic phosphate. This may be a way of recycling phosphate in the cell, and the level of phosphate may be elevated under conditions of phosphate starvation (in algae for example). Acid phosphatases have pH optima below 7, whereas alkaline phosphatases are most active above pH 7. Commercial quantities of acid phosphatase are obtained from plant sources e.g. potato and wheat germ, whereas similar quantities of alkaline phosphatase are obtained from animal intestine and bacteria.

Function:
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.

Subunit:
Isoform 1 interacts with the SH3 domain of SPTAN1. There is no interaction observed for isoforms 2 or 3. Interacts with EPHA2; dephosphorylates EPHA2. Interacts with EPHB1.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
T-lymphocytes express only isoform 2.

Similarity:
Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

SWISS:
P24666

Gene ID:
52

Database links:

Entrez Gene: 52 Human

Entrez Gene: 11431 Mouse

Entrez Gene: 631286 Mouse

Entrez Gene: 24161 Rat

Omim: 171500 Human

SwissProt: P24666 Human

SwissProt: Q9D358 Mouse

SwissProt: P41498 Rat

Unigene: 558296 Human

Unigene: 359831 Mouse

Unigene: 108187 Rat

Unigene: 202830 Rat

Unigene: 220315 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Acid Phosphatase) Polyclonal Antibody, Unconjugated (bs-6291R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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