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RNF45 Rabbit pAb (bs-6511R)  
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產品編號 bs-6511R
英文名稱 RNF45 Rabbit pAb
中文名稱 環指蛋白45/自分泌運動因子受體抗體
別    名 AMF receptor; AMF receptor isoform 1; AMF receptor isoform 2; AMFR; AMFR2_HUMAN; Autocrine motility factor receptor; Autocrine motility factor receptor precursor, isoform 1; Autocrine motility factor receptor precursor, isoform 2; E3 ubiquitin-protein ligase AMFR; gp78; RING finger protein 45; RNF 45; RNF45.  
研究領域 腫瘤  細胞生物  腫瘤細胞生物標志物  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 73 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human AMFR/RNF45: 85-180/643 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 E3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.

Subcellular Location:
Endoplasmic reticulum membrane.

Similarity:
Contains 1 CUE domain.
Contains 1 RING-type zinc finger.

SWISS:
Q9UKV5

Gene ID:
267

Database links:

Entrez Gene: 267 Human

Entrez Gene: 23802 Mouse

Omim: 603243 Human

SwissProt: Q9UKV5 Human

SwissProt: Q9R049 Mouse

Unigene: 295137 Human

Unigene: 34641 Mouse



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF45) Polyclonal Antibody, Unconjugated (bs-6511R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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