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ADM2 Rabbit pAb (bs-2985R)  
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產品編號 bs-2985R
英文名稱 ADM2 Rabbit pAb
中文名稱 中介素抗體
別    名 ADM2; IMD; Intermedin; Adrenomedullin-2; AM2; IMDL; IMDS; ADM2_HUMAN; Adrenomedullin 2; dJ579N16.4; FLJ21135; IMDL; Intermedin-long; Intermedin-short.  
Specific References  (2)     |     bs-2985R has been referenced in 2 publications.
[IF=4.24] Nagasaki, Shuji, et al. "Induction of adrenomedullin2/intermedin expression by thyroid stimulating hormone in thyroid." Molecular and Cellular Endocrinology (2014).  IHC-P ;  Rat.  
[IF=2.27] M?ller, Martin Nue, et al. "The human endolymphatic sac expresses natriuretic peptides." The Laryngoscope (2017).  IHC ;  Human.  
研究領域 心血管  細胞生物  免疫學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 5.2 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Intermedin: 75-148/148 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a protein which is a member of the calcitonin-related hormones. The encoded protein is involved in maintaining homeostasis in many tissues, acting via CRLR/RAMP receptor (calcitonin receptor-like receptor/receptor activity-modifying protein) complexes. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011].
TISSUE SPECIFICITY:High expression detected in the submaxillary gland, kidney, stomach, and mesentery, followed by the pituitary, lung, pancreas, intestines, spleen, thymus and ovary. Expressed mainly in the intermediate lobe of the pituitary, with sporadic in the anterior lobe.

Function:
IMDL and IMDS may play a role as physiological regulators of gastrointestinal, cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent pathway.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed in the esophagus, stomach, jejunum, ileum, ileocecum, ascending colon, transverse colon, descending colon and rectum. Expressed in myocardial cells of the heart, renal tubular cells, hypothalamus, and pituitary.

Similarity:
Belongs to the adrenomedullin family.

SWISS:
Q7Z4H4

Gene ID:
79924

Database links:

Entrez Gene: 79924 Human

Entrez Gene: 223780 Mouse

Entrez Gene: 399475 Rat

Omim: 608682 Human

SwissProt: Q7Z4H4 Human

SwissProt: Q7TNK8 Mouse

SwissProt: P61312 Rat

Unigene: 449099 Human



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADM2) Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
human gastric carcinomaParaformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADM2) Polyclonal Antibody, Unconjugated (bs-2985R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ADM2) Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADM2) Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ADM2) Polyclonal Antibody, Unconjugated (bs-2985R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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