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phospho-PLB (Ser16) Rabbit pAb (bs-2984R)  
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產品編號 bs-2984R
英文名稱 phospho-PLB (Ser16) Rabbit pAb
中文名稱 磷酸化心臟磷蛋白抗體
別    名 phospholamban(phospho S16); p-phospholamban(phospho S16); p-PLB(S16); Cardiac phospholamban; CMD1P; PLB; PLN; PPLA_HUMAN.  
產品類型 磷酸化抗體 
研究領域 心血管  免疫學  信號轉導  轉錄調節因子  通道蛋白  細胞表面分子  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 5.7 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Cardiac phospholamban around the phosphorylation site of Ser16: RA(p-S)TI 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Defects in PLN are the cause of cardiomyopathy dilated type 1P (CMD1P) . Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in PLN are the cause of cardiomyopathy familial hypertrophic type 18 (CMH18) . CMH18 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

Function:
Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.

Subunit:
Homopentamer. Interacts with HAX1.

Subcellular Location:
Mitochondrion membrane; Single-pass membrane protein. Sarcoplasmic reticulum.

Tissue Specificity:
Heart.

Post-translational modifications:
Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.

DISEASE:
[DISEASE] Defects in PLN are the cause of cardiomyopathy dilated type 1P (CMD1P) [MIM:609909]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
[DISEASE] Defects in PLN are the cause of familial hypertrophic cardiomyopathy type 18 (CMH18) [MIM:613874]. CMH18 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

Similarity:
Belongs to the phospholamban family.

SWISS:
P26678

Gene ID:
5350

Database links:

Entrez Gene: 5350 Human

Entrez Gene: 18821 Mouse

Entrez Gene: 64672 Rat

Omim: 172405 Human

SwissProt: P26677 Chicken

SwissProt: A4IFH6 Cow

SwissProt: P61012 Dog

SwissProt: P26678 Human

SwissProt: P61014 Mouse

SwissProt: P61013 Pig

SwissProt: P61015 Rabbit

SwissProt: P61016 Rat

Unigene: 170839 Human

Unigene: 745010 Human

Unigene: 34145 Mouse

Unigene: 9740 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-PLB (Ser16)) Polyclonal Antibody, Unconjugated (bs-2984R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-PLB (Ser16)) Polyclonal Antibody, Unconjugated (bs-2984R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-PLB (Ser16)) Polyclonal Antibody, Unconjugated (bs-2984R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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