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AT2A1/SERCA1 ATPase Rabbit pAb (bs-7539R)  
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產品編號 bs-7539R
英文名稱 AT2A1/SERCA1 ATPase Rabbit pAb
中文名稱 肌漿網鈣ATP酶1/內質網鈣ATP酶1抗體
別    名 fast twitch skeletal muscle isoform; AT2A1_HUMAN; ATP2A; ATP2A1; ATPase Ca++transporting cardiac muscle fast twitch 1; ATPase Ca++transporting fast twitch 1; ATPase, Ca(2+)-transporting fast twitch 1; Calcium pump 1; Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform; Calcium-transporting ATPase sarcoplasmic reticulum type; EC 3.6.3.8; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Fast skeletal muscle SR calcium ATPase; OTTHUMP00000162561; OTTHUMP00000162562; Sarcoendoplasmic reticulum calcium ATPase; Sarcoplasmic reticulum Ca(2+)-ATPase 1; Ca-ATPpase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA 1; SERCA1; SERCA1 truncated isoform, included; SR Ca(2+) ATPase 1; SR Ca(2+)-ATPase 1.  
研究領域 心血管  細胞生物  信號轉導  轉錄調節因子  通道蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 110 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SERCA1 ATPase: 101-200/1001 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Function:
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Subunit:
Associated with sarcolipin (SLN) and phospholamban (PLN) (By similarity).

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity:
Skeletal muscle, fast twitch muscle (type II) fibers.

DISEASE:
Defects in ATP2A1 are the cause of Brody myopathy (BRM) [MIM:601003]. An autosomal recessive myopathy characterized by increasing impairment of relaxation of fast twist skeletal muscle during exercise.

Similarity:
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

SWISS:
O14983

Gene ID:
487

Database links:

Entrez Gene: 518117 Cow

Entrez Gene: 487 Human

Entrez Gene: 11937 Mouse

Entrez Gene: 100037716 Rabbit

Entrez Gene: 116601 Rat

Omim: 108730 Human

SwissProt: Q0VCY0 Cow

SwissProt: O14983 Human

SwissProt: Q8R429 Mouse

SwissProt: P04191 Rabbit

SwissProt: Q64578 Rat

Unigene: 657344 Human

Unigene: 35134 Mouse

Unigene: 217139 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AT2A1 Polyclonal Antibody, Unconjugated (bs-7539R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AT2A1 Polyclonal Antibody, Unconjugated (bs-7539R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AT2A1 Polyclonal Antibody, Unconjugated (bs-7539R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AT2A1) Polyclonal Antibody, Unconjugated (bs-7539R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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