| 產品編號 | bs-7541R |
| 英文名稱 | EDG3 Rabbit pAb |
| 中文名稱 | 內皮分化型G蛋白偶聯受體3抗體 |
| 別 名 | EDG 3; EDG3; Endothelial differentiation G protein coupled receptor 3; Endothelial differentiation G-protein coupled receptor 3; Endothelial differentiation gene 3; Endothelial differentiation sphingolipid G protein coupled receptor 3; FLJ37523; FLJ93220; G protein coupled receptor endothelial differentiation gene 3; LPB 3; LPB3; S1P receptor 3; S1P receptor Edg 3; S1P receptor Edg-3; S1P receptor EDG3; S1P3; S1PR3; S1PR3_HUMAN; Sphingosine 1 phosphate receptor 3; Sphingosine 1 phosphate receptor Edg 3; Sphingosine 1 phosphate receptor Edg3; Sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3. |
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Specific References (5) | bs-7541R has been referenced in 5 publications.
[IF=6.117] Arianna Mazzoli. et al. SKELETAL MUSCLE INSULIN RESISTANCE AND ADIPOSE TISSUE HYPERTROPHY PERSIST BEYOND THE RESHAPING OF GUT MICROBIOTA IN YOUNG RATS FED A FRUCTOSE-RICH DIET. J NUTR BIOCHEM. 2022 Dec;:109247 WB ; Rat.
[IF=4.074] Dingkang Xu. et al. Sphingosine‐1‐phosphate receptor 3 is implicated in BBB injury via the CCL2‐CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28 WB,IF,IHC ; Human.
[IF=3.612] Hai B. Tran. et al. Dysregulated zinc and sphingosine-1-phosphate signaling in pulmonary hypertension: Potential effects by targeting of bone morphogenetic protein receptor type 2 in pulmonary microvessels. 2021 Aug 15 IF ; Rat.
[IF=3.388] Xiao et al. Fingolimod Suppresses a Cascade of Core Vicious Cycle in Dry Eye NOD Mouse Model: Involvement of Sphingosine-1-Phosphate Receptors in Infiltrating Leukocytes. (2017) Invest.Ophthalmol.Vis.Sci. 58:6123-6132 IHC ; Mouse.
[IF=2.733] Namiko et al. Up-regulation of Sphingosine-1-phosphate Receptors and Sphingosine Kinase 1 in the Peri-ischemic Area after Transient Middle Cerebral Artery Occlusion in Mice. Brain Res
. 2020 Jul 15;1739:146831. IHC ; mouse.
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| 研究領域 | 細胞生物 信號轉導 轉錄調節因子 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Cow) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2u/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EDG3: 145-250/378 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008]. Function: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q99500 Gene ID: 1903 Database links: Entrez Gene: 1903 Human Entrez Gene: 13610 Mouse Omim: 601965 Human SwissProt: Q99500 Human SwissProt: Q9Z0U9 Mouse Unigene: 585118 Human Unigene: 136736 Mouse Unigene: 23918 Rat |
| 產品圖片 |
Sample: Lane1: Heart (Mouse) Lysate at 40 ug Primary: Anti-EDG3 (bs-7541R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 42 kD Observed band size: 46 kD
Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Lane 4: Heart (Mouse) Lysate at 40 ug
Primary: Anti-EDG3 (bs-7541R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-EDG3 Polyclonal Antibody, Unconjugated(bs-7541R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-EDG3 antibody (bs-7541R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
