| 產品編號 | bs-0295G-Cy5 |
| 英文名稱 | Goat Anti-Rabbit IgG H&L, Cy5 conjugated |
| 中文名稱 | Cy5標記的羊抗兔IgG H&L |
| 別 名 | Goat Anti-Rabbit IgG H&L (Cy5); Immunoglobulin G; |
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Specific References (17) | bs-0295G-Cy5 has been referenced in 17 publications.
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| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rabbit |
| 產品應用 | IF=1:100-1000,Flow-Cyt=1:100-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 2.0 mg/ml |
| 免 疫 原 | Native rabbit IgG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G, nonspecific adsorbed |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AKT1 Monoclonal Antibody, Unconjugated(bsm-52010R) at 1:200 overnight at 4°C, followed by a Cy5 conjugated Goat Anti-Rabbit IgG （bs-0295G-Cy5）antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AKT1 Monoclonal Antibody, Unconjugated(bsm-52010R) at 1:200 overnight at 4°C, followed by a Cy5 conjugated Goat Anti-Rabbit IgG （bs-0295G-Cy5）antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AKT1 Monoclonal Antibody, Unconjugated(bsm-52010R) at 1:200 overnight at 4°C, followed by a Cy5 conjugated Goat Anti-Rabbit IgG （bs-0295G-Cy5）antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
