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FLII Rabbit pAb (bs-7864R)  
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產品編號 bs-7864R
英文名稱 FLII Rabbit pAb
中文名稱 蛋白質flightless-1同源物抗體
別    名 Fli 1; FLI; Flightless-1; Flightless1; Flightless 1; Flightless I(Drosophila) homolog; Flightless I homolog; Flightless I homolog(Drosophila); Flightless1; FlightlessI; Fliih; MGC39265; Protein flightless 1 homolog; FLI1_HUMAN.  
研究領域 細胞生物  免疫學  干細胞  細胞周期蛋白  細胞分化  細胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 51 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Flightless 1: 343-452/452 
亞    型
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene leads to abnormal muscle function, arrested development and embryonic lethality. The protein sequence shows that this might be a regulator of cytoskeleton and may have a role during cell division.

Function:
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Subunit:
Can form homodimers or heterodimers with ETV6/TEL1.

Subcellular Location:
Nucleus.

DISEASE:
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.

Similarity:
Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.

SWISS:
Q13045

Gene ID:
2314

Database links:

Entrez Gene: 2314 Human

Entrez Gene: 14248 Mouse

Entrez Gene: 287375 Rat

Omim: 600362 Human

SwissProt: Q13045 Human

SwissProt: Q9JJ28 Mouse

Unigene: 513984 Human

Unigene: 339755 Mouse

Unigene: 144698 Rat



產品圖片
Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Flightless 1 (bs-7864R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Flightless 1) Polyclonal Antibody, Unconjugated (bs-7864R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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