| 產品編號 | bs-0988R |
| 英文名稱 | TFRC Rabbit pAb |
| 中文名稱 | 轉鐵蛋白受體（CD71）抗體 |
| 別 名 | Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor(p90 CD71); TRFR; TFR1_HUMAN. |
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Specific References (2) | bs-0988R has been referenced in 2 publications.
[IF=10.787] Limin Shi. et al. FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgfβ1 signaling pathway. REDOX BIOL. 2023 Jul;63:102727 WB ; Mouse.
[IF=3.347] Song Q et al. Astragaloside IV protects against retinal iron overload toxicity through iron regulation and the inhibition of MAPKs and NF-κB activationToxicol Appl Pharmacol.2021 Jan 1;410:115361. WB ; Human.
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| 研究領域 | 腫瘤 心血管 細胞生物 免疫學 信號轉導 干細胞 轉錄調節因子 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Cow,Dog,GuineaPig,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,Flow-Cyt=1μg /Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74/85 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Transferrin receptor: 621-720/760 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] Function: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Subunit: Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4. Subcellular Location: Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. Similarity: Belongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain. SWISS: P02786 Gene ID: 7037 Database links: Entrez Gene: 7037 Human Entrez Gene: 22042 Mouse Omim: 190010 Human SwissProt: P02786 Human SwissProt: Q62351 Mouse Unigene: 529618 Human Unigene: 28683 Mouse Unigene: 98672 Rat 轉鐵蛋白及轉鐵蛋白受體(TfnrR)是機體鐵代謝中的重要因子，鐵是細胞生長增殖、代謝的必需元素,轉鐵蛋白受體(TfR)介導的胞吞作用是細胞攝取鐵的主要途徑。腫瘤細胞因增殖生長旺盛,對鐵的需求高,所以腫瘤細胞有比正常細胞更多的轉鐵蛋白受體的表達。 |
| 產品圖片 |
Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-CD71/Transferrin receptor (bs-0988R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74/85 kD
Observed band size: 75 kD
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFRC ) Polyclonal Antibody, Unconjugated (bs-0988R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue):Raji(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- CD71 antibody(bs-0988R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-CD71 antibody (bs-0988R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
