| 產品編號 | bs-9181R |
| 英文名稱 | Repetin Rabbit pAb |
| 中文名稱 | 中間絲相關蛋白抗體 |
| 別 名 | Intermediate filament associated protein; RPTN; RPTN_HUMAN. |
| 研究領域 | 細胞生物 信號轉導 細胞周期蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Cow,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Repetin: 41-140/784 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Repetin is a 784 amino acid secreted protein that belongs to the S100-fused protein family. Repetin is an extracellular epidermal matrix protein that is expressed in the epidermis and at high levels in eccrine sweat glands, the inner sheaths of hair roots and the filiform papilli of the tongue. A multifunctional epidermal matrix protein, repetin is involved in the cornified cell envelope formation. Containing two EF-hand domains, repetin’s recombinant N-terminal domain binds calcium reversibly. Repetin and trichohyalin may compensate for absent loricrin. The repetin gene contains three exons, two introns and spans at least 5.6 kb. The repetin gene is conserved in cow, mouse and rat, and maps to human chromosome 1q21.3. Function: Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium. Subcellular Location: Secreted; extracellular space; extracellular matrix. Tissue Specificity: Repetin is a multifunctional epidermal matrix protein involved in the cornified cell envelope formation. It reversibly binds calcium. Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheat and in the filiform papilli of the tongue. Post-translational modifications: Potential substrate of transglutaminase. Some arginines are probably converted. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the S100-fused protein family. Contains 2 EF-hand domains. SWISS: Q6XPR3 Gene ID: 126638 Database links: Entrez Gene: 126638 Human Entrez Gene: 20129 Mouse Omim: 613259 Human SwissProt: Q6XPR3 Human SwissProt: P97347 Mouse Unigene: 376144 Human |
| 產品圖片 |
Sample:
A431(human) cell Lysate at 40 ug
Primary: Anti- repetin (bs-9181R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91kD
Observed band size: 91 kD
Sample:
Hela(human)cell Lysate at 40 ug
Primary: Anti- repetin (bs-9181R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91kD
Observed band size: 91 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
