| 產品編號 | bs-9320R |
| 英文名稱 | HAUS8 Rabbit pAb |
| 中文名稱 | HAUS8蛋白抗體 |
| 別 名 | HAUS augmin like complex subunit 8; HAUS augmin-like complex subunit 8; Hec1 interacting and centrosome associated 1; HEC1/NDC80 interacting centrosome associated protein 1; HICE 1; HICE1; MGC20533; NY SAR 48; Sarcoma antigen NY SAR 48; NYS48; HAUS8_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 細胞周期蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HAUS8 protein: 166-410/410 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
NYS48 belongs to the HAUS8 family. It is required for stabilizing spindle integrity and chromosomal integrity. Function: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Subunit: Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Associates with microtubules. The interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. It is unclear whether this interaction is direct or indirect. Subcellular Location: Cytoplasm, cytoskeleton, Microtubule. Similarity: Belongs to the HAUS8 family. SWISS: Q9BT25 Gene ID: 93323 Database links:
Entrez Gene: 93323 Human
Omim: 613434 Human
SwissProt: Q9BT25 Human
Unigene: 404088 Human
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| 產品圖片 |
Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Mouse Spleen tissue lysates
Primary: Anti- HAUS8 (bs-9320R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Sample:
Lane 1: Mouse Lymph Lysates
Lane 2: Mouse SpleenLysates
Lane 3: Human Jurkat cell Lysates
Lane 4: Human MOLT4 cell Lysates
Lane 5: Human K562 cell Lysates
Primary: Anti-HAUS8 (bs-9320R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kDa
Observed band size: 45kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
