| 產品編號 | bs-9341R |
| 英文名稱 | MARCH7 Rabbit pAb |
| 中文名稱 | 膜相關環指蛋白7抗體 |
| 別 名 | AXO; AXOT; Axotrophin; E3 ubiquitin-protein ligase MARCH7; MARCH VII; MARCH-VII; March7; MARH7_HUMAN; Membrane associated RING CH protein VII; Membrane associated ring finger(C3HC4) 7 E3 ubiquitin protein ligase; membrane associated ring finger C3HC4 7; Membrane associated RING finger protein 7; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; RING finger protein 177; RNF177. |
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Specific References (5) | bs-9341R has been referenced in 5 publications.
[IF=5.008] Hu, Jianguo, et al. "Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth." Oncotarget 6.14 (2015): 12174. IHC-P ; Human.
[IF=4.743] Xu M et al. Acetate attenuates inflammasome activation through GPR43-mediated Ca 2+-dependent NLRP3 ubiquitination. Exp Mol Med. 2019 Jul 23;51(7):83. WB&IP ; Mouse.
[IF=2.224] Liu L et al. miR-27b-3p/MARCH7 regulates invasion and metastasis of endometrial cancer cells through Snail-mediated pathway. Acta Biochim Biophys Sin (Shanghai). 2019 Apr 22. pii: gmz030. IHC ; Human.
[IF=1.664] Hu et al. Ubiquitin E3 Ligase MARCH7 promotes proliferation and invasion of cervical cancer cells through VAV2-RAC1-CDC42 pathway. (2018) Oncol.Lett. 16:2312-2318 IHC ; Mice.
[IF=1.29] Zhang, Li, et al. "Expression of USP7 and MARCH7 Is Correlated with Poor Prognosis in Epithelial Ovarian Cancer." The Tohoku Journal of Experimental Medicine 239.3 (2016): 165-175. IHC-P ; Human.
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| 研究領域 | 細胞生物 神經生物學 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 細胞外基質 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MARCH7/Axotrophin: 31-130/704 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum. Function: E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Similarity: Contains 1 RING-CH-type zinc finger. SWISS: Q9H992 Gene ID: 64844 Database links: Entrez Gene: 64844 Human Entrez Gene: 57438 Mouse Omim: 613334 Human SwissProt: Q9H992 Human SwissProt: Q9WV66 Mouse SwissProt: Q5R9W2 Orangutan Unigene: 529272 Human Unigene: 260635 Mouse Unigene: 117620 Rat |
| 產品圖片 |
Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 78/74 kD
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
