| 產品編號 | bs-8689R |
| 英文名稱 | MEPE Rabbit pAb |
| 中文名稱 | 細胞外基質磷酸化抗體 |
| 別 名 | Matrix extracellular phosphoglycoprotein; MEPE; MEPE_HUMAN; OF45; Osteoblast/osteocyte factor 45. |
 |
Specific References (1) | bs-8689R has been referenced in 1 publications.
[IF=3.423] Baris Ozgur Donmez. et al. Calcium?dependent activation of PHEX, MEPE and DMP1 in osteocytes. MOL MED REP. 2022 Dec;26(6):1-11 WB ; Mouse.
|
| 研究領域 | 免疫學 發育生物學 信號轉導 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MEPE: 201-300/525 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
MEPE is a 525 amino acid extracellular matrix protein. Expressed in osteocytes and brain, MEPE is a regulator of bone metabolism that is thought to mediate mineralization and demineralization within the osteocyte microenvironment. MEPE contains an RGD cell-attachment motif and shares molecular similarities with several dentin-bone extracellular matrix RGD-containing phosphoglycoproteins, including OPN (osteopontin) and DSP (dentin sialophosphoprotein). Via its ability to control bone mineralization, MEPE is associated with various developmental events such as skeletogenesis, bone regeneration and odontogenesis. MEPE is secreted in hypophosphatemic osteomalacia tumors, suggesting a possible role in the pathophysiology of bone-related cancers. Function: Seems to play a role in mineralization. Subcellular Location: Secreted Tissue Specificity: Expressed by osteoblasts. Secreted from oncogenic hypophosphataemic tumors. Post-translational modifications: Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. SWISS: Q9NQ76 Gene ID: 56955 Database links: Entrez Gene: 56955 Human Entrez Gene: 94111 Mouse Omim: 605912 Human SwissProt: Q9NQ76 Human Unigene: 676357 Human Unigene: 196672 Mouse Unigene: 160717 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Spleen tissue lysates
Lane 4: Mouse Cerebellum tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human MCF-7 cell lysates
Primary: Anti-MEPE (bs-8689R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 47,62 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
