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MPO Rabbit pAb (bs-4943R)  
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50ul/1180.00元
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產品編號 bs-4943R
英文名稱 MPO Rabbit pAb
中文名稱 髓過氧化物酶抗體
別    名 Myeloperoxidase; MPO; 89 kDa myeloperoxidase; 84 kDa yeloperoxidase; Myeloperoxidase heavy chain; EC 1.11.1.7; PERM_HUMAN.  
Specific References  (17)     |     bs-4943R has been referenced in 17 publications.
[IF=11.53] Ye, Buqing. et al. Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity. Cell Mol Immunol. 2022 Mar;:1-15  IF ;  E.Coli.  
[IF=5.595] Chen B et al. A self-organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide-induced acute lung injury.FASEB J. 2018 Jun 7:fj201701506RR.  ICC ;  Human.  
[IF=4.755] Bae Ju-Eun. et al. Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model. FRONT PHYSIOL. 2022 Oct;0:2117  IHC ;  Mouse.  
[IF=4.556] Tae-Young Kim. et al. Facilitation of Bone Healing Processes Based on the Developmental Function of Meox2 in Tooth Loss Lesion. Int J Mol Sci. 2020 Jan;21(22):8701  IHC ;  Mouse.  
[IF=4.249] Zha C et al. Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation.Neuropharmacology. 2018 Aug;138:140-150.  ICF ;  Human.  
[IF=4.129] Yin Xian. et al. Sleeve Gastrectomy Attenuates the Severity of Cerulein-Induced Acute Pancreatitis in Obese Rats. 2021 Jun 21  IF ;  Rat.  
[IF=3.74] Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.  IHC-P ;  Rat.  
[IF=3.585] Li Y et al. Role of intestinal microbiota-mediated genipin dialdehyde intermediate formation in geniposide-induced hepatotoxicity in rats. Toxicol Appl Pharmacol. 2019 Jun 11;377:114624.  IHC-P ;  Rat.  
[IF=3.484] Boon Ching Teeet al. Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration. Xenotransplantation . 2020 Sep;27(5):e12625.  IHC ;  rat.  
[IF=3.338] Li Yue. et al. Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide. J ANIM SCI. 2022 Oct;:  IF ;  Pig.  
[IF=3.201] Chuntong Bao. et al. IFN-γ-/- mice resist Actinobacillus pleuropneumoniae infection by promoting early lung IL-18 release and PMN-I accumulation. Infect Immun. 2021 Mar;:IAI.00069-21  IF ;  Mouse.  
[IF=2.575] Zhang?H et al. Lentivirus-mediated knockdown of FcγRI (CD64) attenuated lupus nephritis via inhibition of NF-κB regulating NLRP3 inflammasome activation in MRL/lpr mice. J Pharmacol Sci. 2018 Aug;137(4):342-349.   IHF-P ;  Mouse.  
[IF=2.525] Thais Fantozzi,et al.Acute lung injury induced by intestinal ischemia and reperfusion is altered in obese female mice.(2018) Pulmonary Pharmacology & Therapeutics. 49:54-59.  IHC ;  Mouse.  
[IF=1.834] Uygar Sa??k. et al. Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats. BIOTECH HISTOCHEM. 2022 Aug 23  IHC ;  Rat.  
[IF=1.56] Hu, Hai Xia, et al. "Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. Corrigendum in/mmr/12/3/3998." Molecular medicine reports 12.1 (2015): 1321-1327.  IHC-P ;  Rat.  
[IF=1.36] Sekerci,et al.Apelin/APJ expression in the heart and kidneys of hypertensive rats.(2018) Acta Histochemica. 120:196-204.  IHC-P + WB ;  Rat.  
[IF=0] Kurniati NF et al. Myocardial Infarction Elevates Inflammation and Contributes to the Formation of Atheroma Plaques in the Aorta of Hypercholesterolaemic Rats.Makara J. Health Res., 2018, 22(1): 40-45.  WB ;  Rat.  
研究領域 腫瘤  心血管  細胞生物  免疫學  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse (predicted: Rat,Rabbit,Dog,GuineaPig,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 84 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Myeloperoxidase heavy chain: 678-745/745 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Function:
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Subunit:
Tetramer of two light chains and two heavy chains.

Subcellular Location:
Lysosome.

DISEASE:
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

Similarity:
Belongs to the peroxidase family. XPO subfamily.

SWISS:
P05164

Gene ID:
4353

Database links:

Entrez Gene: 4353 Human

Entrez Gene: 17523 Mouse

Entrez Gene: 303413 Rat

Omim: 606989 Human

SwissProt: P05164 Human

SwissProt: P11247 Mouse

Unigene: 458272 Human

Unigene: 4668 Mouse

Unigene: 47782 Rat



髓過氧化物酶(myeloperoxidase,MPO),是一種血紅素蛋白,富含于中性粒細胞中,由粒細胞進入循環之前在骨髓內合成并存儲于噬天青顆粒內。外界刺激可導致中性粒細胞聚集,從而釋放髓過氧化物酶。MPO的相對分子量為150kDa,是由兩個亞單位通過共價結合形成的四聚體,每個亞單位又有一條重鏈α(相對分子量60kDa)和一條輕鏈β鏈(相對分子量為15kDa)構成。(重鏈和輕鏈通過二硫鍵結合)
產品圖片
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MPO Polyclonal Antibody, Unconjugated(bs-4943R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MPO) Polyclonal Antibody, Unconjugated (bs-4943R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MPO Polyclonal Antibody, Unconjugated(bs-4943R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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