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phospho-SAPK3 (Thr183+Tyr185) Rabbit pAb (bs-12001R)  
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50ul/1180.00元
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200ul/2800.00元
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產品編號 bs-12001R
英文名稱 phospho-SAPK3 (Thr183+Tyr185) Rabbit pAb
中文名稱 磷酸化絲裂原活化蛋白激酶磷酸酶p38γ抗體
別    名 p-SAPK3(phospho-Thr183+Tyr185); MAP kinase 12; MAP kinase p38 gamma; MAPK 12; Mapk12; Mitogen Activated Protein Kinase 12; ERK6; ERK 6; ERK-6; Extracellular signal-regulated kinase 6; Mitogen activated protein kinase 3; Mitogen activated protein kinase p38 gamma; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase p38 gamma; MK12_HUMAN; P38 GAMMA; P38GAMMA; PRKM12; SAPK 3; SAPK3; Stress Activated Protein Kinase 3; Stress-activated protein kinase 3.  
產品類型 磷酸化抗體 
研究領域 細胞生物  信號轉導  細胞凋亡  激酶和磷酸酶  細胞粘附分子  細胞分化  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,GuineaPig)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 42 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human SAPK3 around the phosphorylation site of Thr183+Tyr185: EM(p-T)G(p-Y)VV 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38 alpha, p38 beta and p38 gamma, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38 alpha, p38 beta and p38 gamma are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.

Function:
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.

Subcellular Location:
Cytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5.

Tissue Specificity:
Highly expressed in skeletal muscle and heart.

Post-translational modifications:
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
MAP kinase subfamily.
Contains 1 protein kinase domain.

SWISS:
P53778

Gene ID:
6300

Database links:

Entrez Gene: 6300 Human

Entrez Gene: 29857 Mouse

Omim: 602399 Human

SwissProt: P53778 Human

SwissProt: O08911 Mouse

Unigene: 432642 Human

Unigene: 38343 Mouse



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