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Kindlin 2 Rabbit pAb (bs-12371R)  
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產品編號 bs-12371R
英文名稱 Kindlin 2 Rabbit pAb
中文名稱 絲裂原誘導蛋白2抗體
別    名 FLJ34213; FLJ44462; UNC112; FERM2_HUMAN; Fermitin family homolog 2; Fermt2; KIND2; Kindlin-2; MIG-2; MIG2; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; Pleckstrin homology domain containing family C(with FERM domain) member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; UNC112B.  
研究領域 腫瘤  細胞生物  發育生物學  信號轉導  干細胞  細胞粘附分子  細胞骨架  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 78 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Kindlin 2/MIG2: 352-420/680 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Mig-2 recruits migfilin to cell-matrix adhesions, while the interaction with filamin mediates the association of migfilin with actin filaments. Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation. Mig-2 expression is transcriptionally elevated in leiomyomas and could be involved in its hormone-mediated growth of leiomyomas of the uterus. Expression of Mig-2 is ubiquitous, and it is found in numerous tumor tissues.

Function:
Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

Subunit:
Interacts with FBLP1.

Subcellular Location:
Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Note=Within actin stress fibers at cell-ECM focal adhesion sites.

Tissue Specificity:
Ubiquitous. Found in numerous tumor tissues.

Similarity:
Belongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain.

SWISS:
Q96AC1

Gene ID:
10979

Database links:

Entrez Gene: 10979 Human

Entrez Gene: 218952 Mouse

Entrez Gene: 289992 Rat

Entrez Gene: 616981 Cow

Omim: 607746 Human

SwissProt: Q96AC1 Human

SwissProt: Q8CIB5 Mouse

Unigene: 509343 Human

Unigene: 210018 Mouse

Unigene: 7658 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kindlin 2) Polyclonal Antibody, Unconjugated (bs-12371R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kindlin 2) Polyclonal Antibody, Unconjugated (bs-12371R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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