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ARF1 Rabbit pAb (bs-12508R)  
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產品編號 bs-12508R
英文名稱 ARF1 Rabbit pAb
中文名稱 ADP核糖基化因子1抗體
別    名 ADP Ribosylation Factor 1; ADP-ribosylation factor 1; ARF 1; ARF-1; ARF1_HUMAN; DJ133P16.1.  
研究領域 細胞生物  信號轉導  轉運蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Cow,Chicken)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 21 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ARF1: 35-130/181 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.

Function:
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.

Subunit:
Interacts with PRKCABP, GGA1 and PIP5K1B (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with ASAP2, HERC1 and ARHGAP21. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. This interaction at the plasma membrane requires a myristoylated NCS1/FREQ. Interacts with TMED2 and TMED10. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for the FAPP2-mediated glucosylceramide transfer activity.

Subcellular Location:
Golgi apparatus. Cytoplasm > perinuclear region.

Similarity:
Belongs to the small GTPase superfamily. Arf family.

SWISS:
P84077

Gene ID:
375

Database links:

Entrez Gene: 375 Human

Entrez Gene: 11840 Mouse

Entrez Gene: 64310 Rat

Omim: 103180 Human

SwissProt: P84077 Human

SwissProt: P84078 Mouse

SwissProt: P84079 Rat

Unigene: 286221 Human

Unigene: 371546 Mouse

Unigene: 6836 Mouse

Unigene: 93735 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ARF1) Polyclonal Antibody, Unconjugated (bs-12508R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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