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Arginyl tRNA synthetase Rabbit pAb (bs-12516R)  
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產品編號 bs-12516R
英文名稱 Arginyl tRNA synthetase Rabbit pAb
中文名稱 精氨酰tRNA合成酶抗體
別    名 ArgRS; There are 2 isoforms produced by alternative initiation.Alternative names2610011N19Rik; 2610037E21Rik; AL033339; Arginine tRNA ligase 1, cytoplasmic; Arginine tRNA ligase; Arginine--tRNA ligase; Arginyl tRNA synthetase 1; Arginyl tRNA synthetase cytoplasmic; Arginyl tRNA synthetase, cytoplasmic; Arginyl-tRNA synthetase, cytoplasmic; ArgRS; AW985894; DALRD 1; DALRD1; EC 6.1.1.19; MGC8641; MGC86730; RARS; SYRC_HUMAN; wu:fa96g09; zgc:56050; zgc:86730.  
研究領域 細胞生物  信號轉導  轉運蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 75 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Arginyl tRNA synthetase: 401-500/660 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]

Function:
Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.

Subunit:
Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts (via N-terminus) with LARS2 (via C-terminus). Monomer; also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.

Subcellular Location:
Cytoplasm.

Similarity:
Belongs to the class-I aminoacyl-tRNA synthetase family.

SWISS:
P54136

Gene ID:
5917

Database links:

Entrez Gene: 5917 Human

Entrez Gene: 104458 Mouse

Entrez Gene: 287191 Rat

Omim: 107820 Human

SwissProt: P54136 Human

SwissProt: Q9D0I9 Mouse

SwissProt: P40329 Rat

Unigene: 654907 Human

Unigene: 284906 Mouse

Unigene: 18711 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Arginyl tRNA synthetase) Polyclonal Antibody, Unconjugated (bs-12516R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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