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Galectin 10 Rabbit pAb (bs-13266R)  
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50ul/1180.00元
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產品編號 bs-13266R
英文名稱 Galectin 10 Rabbit pAb
中文名稱 半乳糖凝集素10抗體
別    名 BSF3; Charcot Leyden crystal protein; Charcot-Leyden crystal protein; CLC; EC 3.1.1.5; Eosinophil lysophospholipase; Gal-10; Galectin-10; LGALS10; LGALS10A; LPPL HUMAN; LPPL_HUMAN; Lysolecithin acylhydrolase; MGC149659.  
研究領域 腫瘤  細胞生物  信號轉導  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Rat
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 16 kDa
檢測分子量
細胞定位 細胞漿 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Galectin 10: 51-142/142 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Charcot-Leyden crystals are endogenous hexagonal bipyramidal crystals present in human tissues and secretions. Presence of Charcot-Leyden crystals correlates with the increased numbers of peripheral blood or tissue eosinophils that occurs with parasitic and allergic processes. Galectin-10, also referred to as Charcot-Leyden crystal (CLC) protein, singularly makes up these crystals. Galectin-10, a member of the galectin family of beta-Galactoside binding proteins that bind to S-type animal lectins, is expressed solely in eosinophilic and basophilic leukocytes. Galectin-10 may possess carbohydrate or IgE-binding activities, and it plays a functional role in the biology of inflammation. Expression of galectin-10 is transcriptionally induced by butyric acid.

Function:
May have both lysophospholipase and carbohydrate-binding activities.

Subcellular Location:
Cytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell.

Tissue Specificity:
Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils.

Similarity:
Contains 1 galectin domain.

SWISS:
Q05315

Gene ID:
1178

Database links:

Entrez Gene: 1178 Human

Omim: 153310 Human

SwissProt: Q05315 Human

Unigene: 889 Human



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 10) Polyclonal Antibody, Unconjugated (bs-13266R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 10) Polyclonal Antibody, Unconjugated (bs-13266R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Galectin 10 Polyclonal Antibody, Unconjugated(bs-13266R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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