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phospho-Syntaxin 1a (Ser188) Rabbit pAb (bs-10315R)  
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產(chǎn)品編號 bs-10315R
英文名稱 phospho-Syntaxin 1a (Ser188) Rabbit pAb
中文名稱 磷酸化突觸融合蛋白1抗體
別    名 Syntaxin 1a(phospho S188); Syntaxin 1a(phospho Ser188); p-Syntaxin 1a(Ser188); HPC 1; Neuron specific antigen HPC1; Neuron-specific antigen HPC-1; OTTHUMP00000174615; OTTHUMP00000174616; OTTHUMP00000174617; OTTHUMP00000174618; P35-1; STX1; STX1A; STX1A_HUMAN; SYN1A; Syntaxin 1A(brain); Syntaxin 1A brain; Syntaxin-1A; Syntaxin-1A.  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  轉(zhuǎn)運蛋白  結(jié)合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Dog,GuineaPig,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 33 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Syntaxin 1a around the phosphorylation site of Ser188: SI(p-S)KQ 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Syntaxin 1a is potentially involved in docking of synaptic vesicles at presynaptic active zones and may play a critical role in neurotransmitter exocytosis. Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder.

Function:
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.

Subunit:
Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF and LGI3. Interacts with SLC6A4. Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Isoform 2: Secreted (Probable).

Tissue Specificity:
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.

Post-translational modifications:
Phosphorylated by DAPK1.

DISEASE:
Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

Similarity:
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.

SWISS:
Q16623

Gene ID:
6804

Database links:

Entrez Gene: 6804 Human

Entrez Gene: 20907 Mouse

Entrez Gene: 116470 Rat

Omim: 186590 Human

SwissProt: P32850 Cow

SwissProt: Q16623 Human

SwissProt: O35526 Mouse

SwissProt: Q5R4L2 Orangutan

SwissProt: P32851 Rat

Unigene: 647024 Human

Unigene: 6225 Mouse

Unigene: 9943 Rat



產(chǎn)品圖片
Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Syntaxin 1a Polyclonal Antibody, Unconjugated(bs-10315R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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