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Hepatitis C Virus genotype 1a NS5 Rabbit pAb (bs-15456R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包裝/詢價(jià)
產(chǎn)品編號 bs-15456R
英文名稱 Hepatitis C Virus genotype 1a NS5 Rabbit pAb
中文名稱 丙型肝炎病毒1a基因型NS5蛋白抗體
別    名 HCV 1a NS5; Hepatitis C virus genotype 1; Hepatitis C virus NS5A; Non-structural protein 5A.  
研究領(lǐng)域 細(xì)菌及病毒  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) (predicted: Hepatitis C Virus genotype 1a)
產(chǎn)品應(yīng)用 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 7.9 kDa
檢測分子量
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Hepatitis C Virus genotype 1a NS5 : 1-50/74 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 No data available.

Function:
The papain-like proteinase 1 (PL1-PRO) and papain-like proteinase 2 (PL2-PRO) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).
Nsp9 is a ssRNA-binding protein (By similarity).

Subunit:
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).

Subcellular Location:
Non-structural protein 3: Host membrane; Multi-pass membrane protein (Potential).
Non-structural protein 4: Host membrane; Multi-pass membrane protein (Potential).
Non-structural protein 6: Host membrane; Multi-pass membrane protein (Potential).
Non-structural protein 7: Host cytoplasm, host perinuclear region (By similarity). Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
Non-structural protein 8: Host cytoplasm, host perinuclear region (By similarity). Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
Non-structural protein 8: Host cytoplasm, host perinuclear region (By similarity). Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.
Non-structural protein 8: Host cytoplasm, host perinuclear region (By similarity). Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes.

Post-translational modifications:
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).

Similarity:
Belongs to the coronaviruses polyprotein 1ab family.
Contains 1 Macro domain.
Contains 2 peptidase C16 domains.
Contains 1 peptidase C30 domain.

Database links:
UniProtKB/Swiss-Prot: P0C6U5.1

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