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DDX21 Rabbit pAb (bs-14218R)  
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產品編號 bs-14218R
英文名稱 DDX21 Rabbit pAb
中文名稱 DDX21抗體
別    名 Ddx21; DDX21_HUMAN; DEAD box protein 21; Gu alpha; Gu-alpha; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu.  
研究領域 轉錄調節因子  轉運蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Rabbit,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 87 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DDX21: 551-650/783 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq, Jul 2008]

Function:
Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

SWISS:
Q9NR30

Gene ID:
9188

Database links:

Entrez Gene: 9188 Human

Entrez Gene: 781917 Cow

Entrez Gene: 317399 Rat

Omim: 606357 Human

SwissProt: Q9NR30 Human

SwissProt: Q3B8Q1 Rat

Unigene: 223141 Human

Unigene: 162310 Rat



產品圖片
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DDX21 Polyclonal Antibody, Unconjugated(bs-14218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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