| 產品編號 | bs-14522R |
| 英文名稱 | EGR4 Rabbit pAb |
| 中文名稱 | 早期生長反應蛋白4抗體 |
| 別 名 | AT133; EGR4_HUMAN; Early growth response protein 4; Nerve growth factor induced clone C; NGFI C; NGFIC; PAT133. |
| 研究領域 | 細胞生物 神經生物學 轉錄調節因子 鋅指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Pig,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EGR4: 501-589/589 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Egr-1, Egr-2, Egr-3 and Egr-4 are nuclear transcription factors belonging to the Egr C2H2-type zinc-finger protein family and containing three C2H2-type zinc fingers. As immediate early proteins, Egr transcription factors are rapidly induced by diverse extracellular stimuli. They are subject to tight differential control through diverse mechanisms at several levels of regulation: transcriptional; translational and posttranslational (including glycosylation, phosphorylation and redox) mechanisms; and protein-protein interaction. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (Egr-site), thereby activating transcription of target genes whose products are required for mitogenisis and differentiation. Egr-2 binds specific DNA sites located in the promoter region of HoxA4. Egr-2 defects cause congenital hypomyelination neuropathy (also designated Charcot-Marie-Tooth disease) and Dejerine-Sottas neuropathology (also designated hereditary motor and sensory neuropathy III). Egr-3 is involved in muscle spindle development and is expressed in T cells 20 minutes following activation. Egr-4 binds to the Egr consensus motif GCGTGGGCG, functions as a transcriptional repressor, and displays autoregulatory activities, downregulating its own gene promoter in a dose dependent manner. Function: EGR4(Early Growth Response protein 4) is a zinc-finger transcription factor that activates the transcription of genes whose products are required for mitogenesis and differentiation. EGR4 is closely related to nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR4 functionally cooperate with NFAT proteins and induce expression of IL2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression. Subcellular Location: Nuclear Similarity: Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: Q05215 Gene ID: 1961 Database links: Entrez Gene: 1961 Human Entrez Gene: 13656 Mouse Omim: 128992 Human SwissProt: Q05215 Human SwissProt: Q9WUF2 Mouse Unigene: 3052 Human |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
