| 產品編號 | bs-16151R |
| 英文名稱 | FMNL3 Rabbit pAb |
| 中文名稱 | FMNL3蛋白抗體 |
| 別 名 | FHOD3; FMNL3_HUMAN; Formin homology 2 domain containing protein 3; Formin like protein 3; WBP3; WW domain binding protein 3. |
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Specific References (2) | bs-16151R has been referenced in 2 publications.
[IF=4.966] Pan ZN et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J. 2020 Jul;34(7):9615-9627. IP ; Mouse.
[IF=4.966] Pan Z et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J
. 2020 Jul;34(7):9615-9627. WB ; Mouse.
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| 研究領域 | 細胞生物 免疫學 信號轉導 結合蛋白 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 117 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FMNL3: 951-1028/1028 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene contains a formin homology 2 domain and has high sequence identity to the mouse Wbp3 protein. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Function: FMNL3 is an actin-binding protein that contains a formin homology 2 domain. Subunit: Interacts with SRGAP2 (via SH3 domain). Subcellular Location: Cytoplasm. Note=Enriched in lamellipodia. Similarity: Belongs to the formin homology family. Contains 1 DAD (diaphanous autoregulatory) domain. Contains 1 FH2 (formin homology 2) domain. Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. SWISS: Q8IVF7 Gene ID: 91010 Database links: Entrez Gene: 91010 Human SwissProt: Q8IVF7 Human Unigene: 179838 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (bs-16151R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti- FMNL3/FITC Conjugated antibody (bs-16151R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
