| 產品編號 | bs-16773R |
| 英文名稱 | KLHL5 Rabbit pAb |
| 中文名稱 | Kelch樣蛋白5抗體 |
| 別 名 | Kelch(Drosophila) like 5; Kelch like 5(Drosophila); KLHL5_HUMAN; Kelch like 5; Klhl5 kelch like 5(Drosophila); Klhl5 protein; Lymphocyte activation associated protein; Myocardial ischemic preconditioning associated protein 6. |
| 研究領域 | 細胞生物 信號轉導 結合蛋白 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 84 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLHL5: 1-100/755 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The kelch repeat protein family is an actin binding protein. It is characterized by tandemly arranged motifs of about 50 amino acids. Most members of the kelch repeat family were cytoskeletal proteins implicated in various cellular processes, such as actin cytoskeleton interaction, cytoplasmic sequestration of transcription factors and cell morphology. KLHL5 has high identity with Drosophila kelch protein and many other family members. A novel splicing variant of KLHL5, named KLHL5b, has been identified. Subcellular Location: Cytoplasm: cytoskeleton. Tissue Specificity: Expressed in adrenal gland, ovary and thyroid gland and less abundantly in lymph node, prostate, spinal chord, testis and trachea. Similarity: Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats. SWISS: Q96PQ7 Gene ID: 51088 Database links: Entrez Gene: 51088 Human Entrez Gene: 71778 Mouse Omim: 608064 Human SwissProt: Q96PQ7 Human |
| 產品圖片 |
Sample:
Lane 1: Human U-87 MG cell Lysates
Lane 2: Human A549 cell Lysates
Primary: Anti-KLHL5 (bs-16773R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84kDa
Observed band size: 100kDa
Sample: Raw264.7 (Mouse) Lysate at 40 ug
Primary: Anti-KLHL5 (bs-16773R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
