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ODCp Rabbit pAb (bs-16872R)  
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產品編號 bs-16872R
英文名稱 ODCp Rabbit pAb
中文名稱 精氨酸脫羧酶抗體
別    名 L-Arginine decarboxylase; ADC; ADC_HUMAN; Antizyme inhibitor 2; ArgDC; Arginine decarboxylase; AZI2; KIAA1945; L-Arginine carboxy lyase; ODC antizyme inhibitor 2; ODC paralog; ODC-p; ODC-paralogue; ODC1L; ODCp; Ornithine decarboxylase like; Ornithine decarboxylase paralog; Ornithine decarboxylase-like protein.  
研究領域 細胞生物  神經生物學  信號轉導  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 50 kDa
檢測分子量
細胞定位 細胞核 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ODCp: 51-150/460 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 ODCp is a 460 amino acid protein that is expressed in brain and testis. ODCp is a member of the Orn/Lys/Arg decarboxylase class-II family that catalyzes the creation of CO2 and agmatine from L-arginine. Mammalian ODCp differs from the forms expressed in bacteria and plants and shares less than 50% homology with Ornithine decarboxylase (ODC). ODCp is associated with the mitochondrial membrane where excess agmatine can be degraded by the enzyme Agmatinase or bound by the imidazoline recepto. In the brain, the highest levels of ODCp are found in the hypothalamus. Mammalian ODCp is thermally unstable and can be inhibited by Ca2+, Co2+ and polyamines.

Function:
Decarboxylates L-arginine to agmatine. Truncated splice isoforms probably lack activity.

Subunit:
Interacts with OAZ1, OAZ2 and OAZ3; the interactions stabilize the complex by inhibiting AZIN2 ubiquitination and degradation. Does not form an heterodimer with ODC1 (By similarity). Monomer. Interaction with OAZ1, OAZ2 and OAZ3; the interactions lead to increased ornithine decarboxylase (ODC)activity and decreased rate of ODC1 degradation.

Subcellular Location:
Nucleus.

Tissue Specificity:
Highly expressed in brain. Also expressed in testis.

Similarity:
Belongs to the Orn/Lys/Arg decarboxylase class-II family.

SWISS:
Q96A70

Gene ID:
113451

Database links:

Entrez Gene: 506961 Cow

Entrez Gene: 478144 Dog

Entrez Gene: 113451 Human

Entrez Gene: 242669 Mouse

Entrez Gene: 366473 Rat

Omim: 608353 Human

SwissProt: Q96A70 Human

SwissProt: Q8BVM4 Mouse

Unigene: 101807 Human

Unigene: 215814 Mouse

Unigene: 50921 Rat



產品圖片
Sample: Testis (Mouse)Lysate at 40 ug Primary: Anti-ODCp(bs-16872R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 50kD
Sample: Raji (Human)Cell Lysate at 40 ug Primary: Anti-ODCp(bs-16872R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 50kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODCp) Polyclonal Antibody, Unconjugated (bs-16872R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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