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OTUD5/DUBA Rabbit pAb (bs-17566R)  
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產品編號 bs-17566R
英文名稱 OTUD5/DUBA Rabbit pAb
中文名稱 去泛素酶A抗體
別    名 Deubiquinating enzyme A; Deubiquitinating enzyme A; DKFZp761A052; DUBA; MGC104871; OTTHUMP00000025821; OTU domain containing 5; OTU domain containing protein 5; OTU domain-containing protein 5; OTUD 5; otud5; OTUD5_HUMAN.  
研究領域 細胞生物  轉錄調節因子  表觀遺傳學  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Chicken)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 61 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human OTUD5/DUBA : 501-571/571 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]

Function:
Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production.

Subunit:
Interacts with TRAF3.

Tissue Specificity:
Expressed in various tissues, including the liver and placenta, as well as in peripheral blood leukocytes.

Post-translational modifications:
Phosphorylation at Ser-177 is required for deubiquitinating activity.

Similarity:
Belongs to the peptidase C85 family.
Contains 1 OTU domain.

SWISS:
Q96G74

Gene ID:
55593

Database links:

Entrez Gene: 55593 Human

Entrez Gene: 54644 Mouse

Entrez Gene: 363452 Rat

Omim: 300713 Human

SwissProt: Q96G74 Human

SwissProt: Q3U2S4 Mouse

SwissProt: Q2YDU3 Rat

Unigene: 496098 Human

Unigene: 142827 Mouse

Unigene: 97794 Rat



產品圖片
Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OTUD5 Polyclonal Antibody, Unconjugated(bs-17566R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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