| 產品編號 | bs-19732R |
| 英文名稱 | RASA4 Rabbit pAb |
| 中文名稱 | GTP酶激活蛋白樣RASA4抗體 |
| 別 名 | Ca2+promoted Ras inactivator; Calcium promoted Ras inactivator; CAPRI; GAPL; RASL2_HUMAN; GTPase activating protein like; KIAA0538; MGC131890; Ras GTPase activating protein 4; RAS p21 protein activator 4; RasGAP activating like protein 2. |
| 研究領域 | 細胞生物 信號轉導 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RASA4: 501-600/803 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Ca2+-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis. Subcellular Location: Cytoplasm; cytosol. Cell membrane; peripheral membrane protein. Note=Localized to the cytosol as a result of its lack of phosphoinositide binding activity. Upon agonist-stimulated calcium mobilization, utilizes the C2A and C2B domains to associate with the plasma membrane. Tissue Specificity: Widely expressed. Similarity: Contains 1 Ras-GAP domain. SWISS: O43374 Gene ID: 10156 Database links: Entrez Gene: 10156 Human Entrez Gene: 54153 Mouse Omim: 607943 Human SwissProt: O43374 Human SwissProt: Q6PFQ7 Mouse Unigene: 696339 Human Unigene: 290655 Mouse Unigene: 1366 Rat |
| 產品圖片 |
Sample:Spleen (Mouse)Lysate at 40 ug
Primary: Anti-RASA4(bs-19732R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 90kD
Observed band size: 90kD
Sample:
HepG2 Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Lovo Cell (Human) Lysate at 40 ug
Primary: Anti-RASA4(bs-19732R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90kD
Observed band size: 90kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
