| 產品編號 | bs-19984R |
| 英文名稱 | PRDM13 Rabbit pAb |
| 中文名稱 | PRDM鋅指轉錄因子PRDM13抗體 |
| 別 名 | MU MB 20.220; PFM 10; PFM10; PR domain containing 13; PR domain containing protein 13; PR domain zinc finger protein 13; PRDM 13. |
| 研究領域 | 細胞生物 轉錄調節因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Hamster (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRDM13: 551-650/707 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
PRDM13 may be involved in transcriptional regulation. Function: May be involved in transcriptional regulation. Subcellular Location: Nuclear Similarity: Belongs to the class V-like SAM-binding methyltransferase superfamily. SWISS: Q9H4Q3 Gene ID: 59336 Database links: Entrez Gene: 59336 Human Entrez Gene: 230025 Mouse SwissProt: Q9H4Q3 Human SwissProt: B1AV74 Mouse Unigene: 287386 Human Unigene: 208541 Mouse |
| 產品圖片 |
Sample:
CHO(Hamster) Cell Lysate at 30 ug
Primary: Anti-PRDM13 (bs-19984R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kD
Observed band size: 74 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRDM13) Polyclonal Antibody, Unconjugated (bs-19984R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
