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PDLIM2 Rabbit pAb (bs-12600R)  
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產品編號 bs-12600R
英文名稱 PDLIM2 Rabbit pAb
中文名稱 PDLIM2蛋白抗體
別    名 FLJ34715; MYSTIQUE; OTTHUMP00000162522; OTTHUMP00000205197; OTTHUMP00000205198; OTTHUMP00000205199; OTTHUMP00000205200; OTTHUMP00000205202; OTTHUMP00000205203; PDLI2_HUMAN; Pdlim2; PDZ and LIM domain 2(mystique); PDZ and LIM domain protein 2; PDZ-LIM protein mystique; SLIM; Stat-interacting LIM protein.  
研究領域 腫瘤  細胞生物  信號轉導  結合蛋白  細胞骨架  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Pig,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 37 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PDLIM2: 31-130/352 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Function:
Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.

Subcellular Location:
Cytoplasm > cytoskeleton; Nucleus; Cytoplasm. Nucleus. May be partially nuclear and Cytoplasm > cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin.

Similarity:
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.

SWISS:
Q96JY6

Gene ID:
64236

Database links:


產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDLIM2) Polyclonal Antibody, Unconjugated (bs-12600R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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