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BTN2A1 Rabbit pAb (bs-20473R)  
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產品編號 bs-20473R
英文名稱 BTN2A1 Rabbit pAb
中文名稱 嗜乳脂蛋白2亞型A1抗體
別    名 BK14H9.1; BT2.1; BT2A1_HUMAN; BTF1; BTN2A1; Butyrophilin BTF1; Butyrophilin subfamily 2 member A1; CTA-14H9.1; DJ3E1.1.  
Specific References  (2)     |     bs-20473R has been referenced in 2 publications.
[IF=12.469] Roberto Benelli. et al. Targeting of colorectal cancer organoids with zoledronic acid conjugated to the anti-EGFR antibody cetuximab. J IMMUNOTHER CANCER. 2022 Dec;10(12):e005660  WB, IHC ;  Human.  
[IF=6.575] Jordi Leonardo Castrillo Fernandez. et al. Priming of Colorectal Tumor-Associated Fibroblasts with Zoledronic Acid Conjugated to the Anti-Epidermal Growth Factor Receptor Antibody Cetuximab Elicits Anti-Tumor Vδ2 T Lymphocytes. CANCERS. 2023 Jan;15(3):610  IF,IHC ;  Human.  
研究領域 細胞生物  信號轉導  細胞凋亡  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 57 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human BTN2A1: 101-200/527 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the immunoglobulin superfamily. The gene is located in a cluster of butyrophilin-like genes in the juxta-telomeric region of the major histocompatibility complex on chromosome 6. A pseudogene of this gene has been identified in this cluster. The encoded protein is an integral plasma membrane protein involved in lipid, fatty-acid, and sterol metabolism. Alterations in this gene may be associated with several disease states including metabolic syndrome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Subcellular Location:
Membrane; Single-pass type I membrane protein

Tissue Specificity:
Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney.

Similarity:
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.


SWISS:
Q7KYR7

Gene ID:
11120

Database links:

Entrez Gene: 11120 Human

Omim: 613590 Human

SwissProt: Q7KYR7 Human

Unigene: 159028 Human



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTN2A1) Polyclonal Antibody, Unconjugated (bs-20473R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control:MCF7 cells (blue). Primary Antibody: Rabbit Anti-BTN2A1 antibody(bs-20473R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Blank control: SH-Sy5Y. Primary Antibody (green line): Rabbit Anti-BTN2A1 antibody (bs-20473R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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