| 產品編號 | bs-8732R |
| 英文名稱 | PIAS1 Rabbit pAb |
| 中文名稱 | 激活STAT蛋白抑制因子1抗體 |
| 別 名 | AR interacting protein; DDXBP1; DEAD/H(Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; GBP; Gu binding protein; Gu-binding protein; GU/RH-II; Pias1; PIAS1_HUMAN; Protein inhibitor of activated STAT protein 1; Protein inhibitor of activated STAT, 1; RNA helicase II binding protein; RNA helicase II-binding protein; Zinc finger, MIZ-type containing 3; ZMIZ3. |
| 研究領域 | 轉錄調節(jié)因子 表觀遺傳學 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 81 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PIAS1: 501-600/651 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | |
| 產品圖片 |
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-PIAS1 (bs-8732R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIAS1) Polyclonal Antibody, Unconjugated (bs-8732R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
