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CA9 Mouse mAb (bsm-33082M)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
200ug(PBS only)/5600.00元
大包裝/詢價

產品編號 bsm-33082M
英文名稱 CA9 Mouse mAb
中文名稱 碳酸酐酶9單克隆抗體
別    名 CA IX; CA-IX; Carbonic anhydrase 9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma associated antigen G250; Renal cell carcinoma-associated antigen G250.  
Specific References  (1)     |     bsm-33082M has been referenced in 1 publications.
[IF=5.923] Alena Pastornická. et al. Influence of Paclitaxel and Doxorubicin Therapy of ?III-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat. Int J Mol Sci. 2021 Jan;22(12):6363  IHC ;  Rat.  
研究領域 腫瘤  細胞生物  免疫學  激酶和磷酸酶  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 5H10
交叉反應 Human
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:500-1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 46 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human Carbonic Anhydrase IX (full length) 
亞    型 IgG
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008]

Function:
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

Subunit:
Forms oligomers linked by disulfide bonds.

Subcellular Location:
Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Tissue Specificity:
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Similarity:
Belongs to the alpha-carbonic anhydrase family.

SWISS:
Q16790

Gene ID:
768

Database links:

Entrez Gene: 768 Human

Entrez Gene: 230099 Mouse

Omim: 603179 Human

SwissProt: Q16790 Human

SwissProt: Q8VHB5 Mouse

Unigene: 63287 Human

Unigene: 283682 Mouse



碳酸酐酶9(carbonic anhydrase IX,CA IX)是新發現的碳酸酐酶家族異構體之一,是由酸性氨基酸組成的跨膜糖蛋白,在調控細胞增殖、轉化方面有重要作用。
碳酸酐酶9能催化CO2水解為碳酸和水,參與機體的酸堿平衡,調節細胞內外pH值,易導致腫瘤的生長和轉移,目前也是子宮腫瘤新的標記物。
產品圖片
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CA9) Monoclonal Antibody, Unconjugated (bsm-33082M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Carbonate dehydratase IX) Monoclonal Antibody, Unconjugated (bsm-33082M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Carbonate dehydratase IX) Monoclonal Antibody, Unconjugated (ascites of bsm-33082M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Carbonate dehydratase IX) Monoclonal Antibody, Unconjugated (bsm-33082M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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