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Prostate Specific Antigen Mouse mAb (bsm-41670M)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
200ug(PBS only)/5600.00元
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產品編號 bsm-41670M
英文名稱 Prostate Specific Antigen Mouse mAb
中文名稱 人前列腺特異性抗原單克隆抗體
別    名 antigen, prostate-specific; APS; gamma seminoprotein; gamma-seminoprotein; hK3; Kallikrein 3; Kallikrein 3, (prostate specific antigen); Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; KLK3; KLK3_HUMAN; P 30 antigen; P-30 antigen; P30 antigen; Prostate Specific Antigen; Prostate-specific antigen; PSA; Semenogelase; Seminin.  
研究領域 腫瘤  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 4C4
交叉反應 Human
產品應用 WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
免 疫 原 Prostate Specific Antigen (PSA) Antigen, Native 
亞    型 IgG1
純化方法 affinity purified by Protein A
緩 沖 液 0.01M PBS (pH7.4).
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Function:
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Subunit:
Forms heterodimer with SERPINA5.

Subcellular Location:
Secreted.

Tissue Specificity:
Prostate.

Similarity:
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.

Database links:

Entrez Gene: 354 Human

Omim: 176820 Human

SwissProt: P07288 Human

Unigene: 171995 Human



產品圖片
Sample: Lane 1: Prostate Specific Antigen (PSA) Protein at 500ng Primary: Mouse Anti-PSA Monoclonal Antibody at 1/1000 dilution (Cat. bsm-41670M) Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 34kD Observed band size: 32kD
Sample: Lane 1: Human Prostate Specific Antigen protein Primary: Anti-Prostate Specific Antigen (bsm-41670M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kDa Observed band size: 35 kDa
Paraformaldehyde-fixed, paraffin embedded (Human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (bsm-41670M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (bsm-41670M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Measured by its binding ability in a indirect ELISA. Immobilized Human PSA (Native) at 2 μg/mL (100 μL/well) can bind Anti-Human PSA monoclonal antibody, the minimum detection concentration is 0.1ng/mL
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