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phospho-Histone H3 (Ser10) Mouse mAb (bsm-60078M)  
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50ul/1580.00元
100ul/2500.00元
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產品編號 bsm-60078M
英文名稱 phospho-Histone H3 (Ser10) Mouse mAb
中文名稱 磷酸化組蛋白H3單克隆抗體
別    名 Histone H3(phospho S10); phospho-Histone H3(Ser10); Histone H3(phospho-Ser10); Histone H3(p-Ser10); Histone H3(phospho-S10); H3S10ph; H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD;  
產品類型 磷酸化抗體 
研究領域 染色質和核信號  細胞類型標志物  表觀遺傳學  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Firefly)
產品應用 WB=1:1000-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 15 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10: RK(p-S)TG 
亞    型 IgG
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

SWISS:
Q71DI3

Gene ID:
8968

Database links:

Entrez Gene: 8350 Human

Entrez Gene: 319152 Mouse

Entrez Gene: 679994 Rat

SwissProt: P68431 Human

SwissProt: P68433 Mouse

SwissProt: Q6LED0 Rat



產品圖片
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+ Nocodazole (100ng/ml, 18hr) + Calyculin A (100nM, 1hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 17 kDa
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